suspension cell cultures Search Results


dexamethasone dex  (Chem Impex International)
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Chem Impex International dexamethasone dex
Dexamethasone Dex, supplied by Chem Impex International, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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cell suspension culture  (Lindl GmbH)
90
Lindl GmbH cell suspension culture
Confirmed botanical sources of tormentic acid (currently accepted botanical names (where applicable) according to www.theplantlist.org (accessed on 12 April 2021) are given in square brackets) and initial extrahent type used for elution of TA from plant material.
Cell Suspension Culture, supplied by Lindl GmbH, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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suspension cell cultures of taxus chinensis  (Phyton Biotech)
90
Phyton Biotech suspension cell cultures of taxus chinensis
Confirmed botanical sources of tormentic acid (currently accepted botanical names (where applicable) according to www.theplantlist.org (accessed on 12 April 2021) are given in square brackets) and initial extrahent type used for elution of TA from plant material.
Suspension Cell Cultures Of Taxus Chinensis, supplied by Phyton Biotech, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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96-well plate dedicated for suspension cell culture inertgradetm  (BRAND GMBH)
90
BRAND GMBH 96-well plate dedicated for suspension cell culture inertgradetm
Confirmed botanical sources of tormentic acid (currently accepted botanical names (where applicable) according to www.theplantlist.org (accessed on 12 April 2021) are given in square brackets) and initial extrahent type used for elution of TA from plant material.
96 Well Plate Dedicated For Suspension Cell Culture Inertgradetm, supplied by BRAND GMBH, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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suspension-cultured cells of a. thaliana (l.) heynh. ecotype columbia cell line t87  (BioResource International Inc)
90
BioResource International Inc suspension-cultured cells of a. thaliana (l.) heynh. ecotype columbia cell line t87
Confirmed botanical sources of tormentic acid (currently accepted botanical names (where applicable) according to www.theplantlist.org (accessed on 12 April 2021) are given in square brackets) and initial extrahent type used for elution of TA from plant material.
Suspension Cultured Cells Of A. Thaliana (L.) Heynh. Ecotype Columbia Cell Line T87, supplied by BioResource International Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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a. thaliana mm2d cell suspension cultures  (Bayer Cropscience Deutschland GmbH)
90
Bayer Cropscience Deutschland GmbH a. thaliana mm2d cell suspension cultures
<t>PCD</t> hallmarks in A. <t>thaliana</t> <t>MM2d</t> cell cultures treated with 35 mm H2O2. A, Cell viability measured using trypan blue dye exclusion assay. Untreated and H2O2-treated cells are represented by solid and dashed lines, respectively. Data is the result of three independent experiments, each including 500 cells. B, Effect of H2O2 treatment on chlorophyll concentration. One milliliter of H2O2-treated cell cultures was collected at the indicated times and the chlorophyll amount was calculated according to MacKinney's protocol (29). C, Effect of H2O2 treatment on protein concentration. Similar to (B), but the protein amount was determined using the Bradford assay (28). D, Bright-field microscope image showing cells treated with H2O2 for 6 h. Cells were stained with trypan blue dye. E, Similar to (D), but after 24 h of H2O2 treatment. F, Bright-field microscope image for 35 mm H2O2 treated cells, stained with trypan blue dye indicating a cell wall (CW) and cell shrinkage (CS). G–J, Changes in the cellular morphology of H2O2-treated cells analyzed by DAPI nuclear staining, chlorophyll fluorescence and bright-field images. Cells were observed under fluorescence (Left Panels G and I) and bright-field (Right panels H and J) microscopy. Upper (G and H) and lower (I and J) panels correspond to 0 and 24 h of H2O2 treatment, respectively. Apoptotic nuclei are stained in blue dye and, having undergone shrinkage, lack red fluorescence.
A. Thaliana Mm2d Cell Suspension Cultures, supplied by Bayer Cropscience Deutschland GmbH, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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293-t cells  (GeneChoice Inc)
90
GeneChoice Inc 293-t cells
<t>PCD</t> hallmarks in A. <t>thaliana</t> <t>MM2d</t> cell cultures treated with 35 mm H2O2. A, Cell viability measured using trypan blue dye exclusion assay. Untreated and H2O2-treated cells are represented by solid and dashed lines, respectively. Data is the result of three independent experiments, each including 500 cells. B, Effect of H2O2 treatment on chlorophyll concentration. One milliliter of H2O2-treated cell cultures was collected at the indicated times and the chlorophyll amount was calculated according to MacKinney's protocol (29). C, Effect of H2O2 treatment on protein concentration. Similar to (B), but the protein amount was determined using the Bradford assay (28). D, Bright-field microscope image showing cells treated with H2O2 for 6 h. Cells were stained with trypan blue dye. E, Similar to (D), but after 24 h of H2O2 treatment. F, Bright-field microscope image for 35 mm H2O2 treated cells, stained with trypan blue dye indicating a cell wall (CW) and cell shrinkage (CS). G–J, Changes in the cellular morphology of H2O2-treated cells analyzed by DAPI nuclear staining, chlorophyll fluorescence and bright-field images. Cells were observed under fluorescence (Left Panels G and I) and bright-field (Right panels H and J) microscopy. Upper (G and H) and lower (I and J) panels correspond to 0 and 24 h of H2O2 treatment, respectively. Apoptotic nuclei are stained in blue dye and, having undergone shrinkage, lack red fluorescence.
293 T Cells, supplied by GeneChoice Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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axenic cell-suspension cultures of the hornwort anthoceros agrestis paton  (Marburg GmbH)
90
Marburg GmbH axenic cell-suspension cultures of the hornwort anthoceros agrestis paton
<t>PCD</t> hallmarks in A. <t>thaliana</t> <t>MM2d</t> cell cultures treated with 35 mm H2O2. A, Cell viability measured using trypan blue dye exclusion assay. Untreated and H2O2-treated cells are represented by solid and dashed lines, respectively. Data is the result of three independent experiments, each including 500 cells. B, Effect of H2O2 treatment on chlorophyll concentration. One milliliter of H2O2-treated cell cultures was collected at the indicated times and the chlorophyll amount was calculated according to MacKinney's protocol (29). C, Effect of H2O2 treatment on protein concentration. Similar to (B), but the protein amount was determined using the Bradford assay (28). D, Bright-field microscope image showing cells treated with H2O2 for 6 h. Cells were stained with trypan blue dye. E, Similar to (D), but after 24 h of H2O2 treatment. F, Bright-field microscope image for 35 mm H2O2 treated cells, stained with trypan blue dye indicating a cell wall (CW) and cell shrinkage (CS). G–J, Changes in the cellular morphology of H2O2-treated cells analyzed by DAPI nuclear staining, chlorophyll fluorescence and bright-field images. Cells were observed under fluorescence (Left Panels G and I) and bright-field (Right panels H and J) microscopy. Upper (G and H) and lower (I and J) panels correspond to 0 and 24 h of H2O2 treatment, respectively. Apoptotic nuclei are stained in blue dye and, having undergone shrinkage, lack red fluorescence.
Axenic Cell Suspension Cultures Of The Hornwort Anthoceros Agrestis Paton, supplied by Marburg GmbH, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/suspension+cell+cultures/axenic+cell+suspension+cultures+of+the+hornwort+anthoceros+agrestis+paton/pmc04512192-67-5-26
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suspension cell culture 100-mm plates  (Corning Life Sciences)
90
Corning Life Sciences suspension cell culture 100-mm plates
<t>PCD</t> hallmarks in A. <t>thaliana</t> <t>MM2d</t> cell cultures treated with 35 mm H2O2. A, Cell viability measured using trypan blue dye exclusion assay. Untreated and H2O2-treated cells are represented by solid and dashed lines, respectively. Data is the result of three independent experiments, each including 500 cells. B, Effect of H2O2 treatment on chlorophyll concentration. One milliliter of H2O2-treated cell cultures was collected at the indicated times and the chlorophyll amount was calculated according to MacKinney's protocol (29). C, Effect of H2O2 treatment on protein concentration. Similar to (B), but the protein amount was determined using the Bradford assay (28). D, Bright-field microscope image showing cells treated with H2O2 for 6 h. Cells were stained with trypan blue dye. E, Similar to (D), but after 24 h of H2O2 treatment. F, Bright-field microscope image for 35 mm H2O2 treated cells, stained with trypan blue dye indicating a cell wall (CW) and cell shrinkage (CS). G–J, Changes in the cellular morphology of H2O2-treated cells analyzed by DAPI nuclear staining, chlorophyll fluorescence and bright-field images. Cells were observed under fluorescence (Left Panels G and I) and bright-field (Right panels H and J) microscopy. Upper (G and H) and lower (I and J) panels correspond to 0 and 24 h of H2O2 treatment, respectively. Apoptotic nuclei are stained in blue dye and, having undergone shrinkage, lack red fluorescence.
Suspension Cell Culture 100 Mm Plates, supplied by Corning Life Sciences, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/suspension+cell+cultures/suspension+cell+culture+100+mm+plates/pmc03665318-90-6-12
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suspension cell culture 100-mm plates - by Bioz Stars, 2026-10
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plant cells  (Protalix Inc)
90
Protalix Inc plant cells
<t>PCD</t> hallmarks in A. <t>thaliana</t> <t>MM2d</t> cell cultures treated with 35 mm H2O2. A, Cell viability measured using trypan blue dye exclusion assay. Untreated and H2O2-treated cells are represented by solid and dashed lines, respectively. Data is the result of three independent experiments, each including 500 cells. B, Effect of H2O2 treatment on chlorophyll concentration. One milliliter of H2O2-treated cell cultures was collected at the indicated times and the chlorophyll amount was calculated according to MacKinney's protocol (29). C, Effect of H2O2 treatment on protein concentration. Similar to (B), but the protein amount was determined using the Bradford assay (28). D, Bright-field microscope image showing cells treated with H2O2 for 6 h. Cells were stained with trypan blue dye. E, Similar to (D), but after 24 h of H2O2 treatment. F, Bright-field microscope image for 35 mm H2O2 treated cells, stained with trypan blue dye indicating a cell wall (CW) and cell shrinkage (CS). G–J, Changes in the cellular morphology of H2O2-treated cells analyzed by DAPI nuclear staining, chlorophyll fluorescence and bright-field images. Cells were observed under fluorescence (Left Panels G and I) and bright-field (Right panels H and J) microscopy. Upper (G and H) and lower (I and J) panels correspond to 0 and 24 h of H2O2 treatment, respectively. Apoptotic nuclei are stained in blue dye and, having undergone shrinkage, lack red fluorescence.
Plant Cells, supplied by Protalix Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/suspension+cell+cultures/plant+cell+suspension+cultures/pmc03564370-92-10-1
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plant cells - by Bioz Stars, 2026-10
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suspension cell cultures of the host plant m. sativa  (Baier labs)
90
Baier labs suspension cell cultures of the host plant m. sativa
<t>PCD</t> hallmarks in A. <t>thaliana</t> <t>MM2d</t> cell cultures treated with 35 mm H2O2. A, Cell viability measured using trypan blue dye exclusion assay. Untreated and H2O2-treated cells are represented by solid and dashed lines, respectively. Data is the result of three independent experiments, each including 500 cells. B, Effect of H2O2 treatment on chlorophyll concentration. One milliliter of H2O2-treated cell cultures was collected at the indicated times and the chlorophyll amount was calculated according to MacKinney's protocol (29). C, Effect of H2O2 treatment on protein concentration. Similar to (B), but the protein amount was determined using the Bradford assay (28). D, Bright-field microscope image showing cells treated with H2O2 for 6 h. Cells were stained with trypan blue dye. E, Similar to (D), but after 24 h of H2O2 treatment. F, Bright-field microscope image for 35 mm H2O2 treated cells, stained with trypan blue dye indicating a cell wall (CW) and cell shrinkage (CS). G–J, Changes in the cellular morphology of H2O2-treated cells analyzed by DAPI nuclear staining, chlorophyll fluorescence and bright-field images. Cells were observed under fluorescence (Left Panels G and I) and bright-field (Right panels H and J) microscopy. Upper (G and H) and lower (I and J) panels correspond to 0 and 24 h of H2O2 treatment, respectively. Apoptotic nuclei are stained in blue dye and, having undergone shrinkage, lack red fluorescence.
Suspension Cell Cultures Of The Host Plant M. Sativa, supplied by Baier labs, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/suspension+cell+cultures/suspension+cell+cultures+of+the+host+plant+m++sativa/pm33853246-50-10-19
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rice cell suspension culture line 0c  (BioResource International Inc)
90
BioResource International Inc rice cell suspension culture line 0c
<t>PCD</t> hallmarks in A. <t>thaliana</t> <t>MM2d</t> cell cultures treated with 35 mm H2O2. A, Cell viability measured using trypan blue dye exclusion assay. Untreated and H2O2-treated cells are represented by solid and dashed lines, respectively. Data is the result of three independent experiments, each including 500 cells. B, Effect of H2O2 treatment on chlorophyll concentration. One milliliter of H2O2-treated cell cultures was collected at the indicated times and the chlorophyll amount was calculated according to MacKinney's protocol (29). C, Effect of H2O2 treatment on protein concentration. Similar to (B), but the protein amount was determined using the Bradford assay (28). D, Bright-field microscope image showing cells treated with H2O2 for 6 h. Cells were stained with trypan blue dye. E, Similar to (D), but after 24 h of H2O2 treatment. F, Bright-field microscope image for 35 mm H2O2 treated cells, stained with trypan blue dye indicating a cell wall (CW) and cell shrinkage (CS). G–J, Changes in the cellular morphology of H2O2-treated cells analyzed by DAPI nuclear staining, chlorophyll fluorescence and bright-field images. Cells were observed under fluorescence (Left Panels G and I) and bright-field (Right panels H and J) microscopy. Upper (G and H) and lower (I and J) panels correspond to 0 and 24 h of H2O2 treatment, respectively. Apoptotic nuclei are stained in blue dye and, having undergone shrinkage, lack red fluorescence.
Rice Cell Suspension Culture Line 0c, supplied by BioResource International Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Image Search Results


Confirmed botanical sources of tormentic acid (currently accepted botanical names (where applicable) according to www.theplantlist.org (accessed on 12 April 2021) are given in square brackets) and initial extrahent type used for elution of TA from plant material.

Journal: Molecules

Article Title: The Occurrence and Biological Activity of Tormentic Acid—A Review

doi: 10.3390/molecules26133797

Figure Lengend Snippet: Confirmed botanical sources of tormentic acid (currently accepted botanical names (where applicable) according to www.theplantlist.org (accessed on 12 April 2021) are given in square brackets) and initial extrahent type used for elution of TA from plant material.

Article Snippet: Rosaceae , Eriobotrya japonica (Thunb) Lindl. Cell suspension culture (obtained from immature embryos) , 95% Ethanol , [ ] .

Techniques: Extraction, Solvent, Cell Culture, Suspension, Methylation

PCD hallmarks in A. thaliana MM2d cell cultures treated with 35 mm H2O2. A, Cell viability measured using trypan blue dye exclusion assay. Untreated and H2O2-treated cells are represented by solid and dashed lines, respectively. Data is the result of three independent experiments, each including 500 cells. B, Effect of H2O2 treatment on chlorophyll concentration. One milliliter of H2O2-treated cell cultures was collected at the indicated times and the chlorophyll amount was calculated according to MacKinney's protocol (29). C, Effect of H2O2 treatment on protein concentration. Similar to (B), but the protein amount was determined using the Bradford assay (28). D, Bright-field microscope image showing cells treated with H2O2 for 6 h. Cells were stained with trypan blue dye. E, Similar to (D), but after 24 h of H2O2 treatment. F, Bright-field microscope image for 35 mm H2O2 treated cells, stained with trypan blue dye indicating a cell wall (CW) and cell shrinkage (CS). G–J, Changes in the cellular morphology of H2O2-treated cells analyzed by DAPI nuclear staining, chlorophyll fluorescence and bright-field images. Cells were observed under fluorescence (Left Panels G and I) and bright-field (Right panels H and J) microscopy. Upper (G and H) and lower (I and J) panels correspond to 0 and 24 h of H2O2 treatment, respectively. Apoptotic nuclei are stained in blue dye and, having undergone shrinkage, lack red fluorescence.

Journal: Molecular & Cellular Proteomics : MCP

Article Title: New Arabidopsis thaliana Cytochrome c Partners: A Look Into the Elusive Role of Cytochrome c in Programmed Cell Death in Plants *

doi: 10.1074/mcp.M113.030692

Figure Lengend Snippet: PCD hallmarks in A. thaliana MM2d cell cultures treated with 35 mm H2O2. A, Cell viability measured using trypan blue dye exclusion assay. Untreated and H2O2-treated cells are represented by solid and dashed lines, respectively. Data is the result of three independent experiments, each including 500 cells. B, Effect of H2O2 treatment on chlorophyll concentration. One milliliter of H2O2-treated cell cultures was collected at the indicated times and the chlorophyll amount was calculated according to MacKinney's protocol (29). C, Effect of H2O2 treatment on protein concentration. Similar to (B), but the protein amount was determined using the Bradford assay (28). D, Bright-field microscope image showing cells treated with H2O2 for 6 h. Cells were stained with trypan blue dye. E, Similar to (D), but after 24 h of H2O2 treatment. F, Bright-field microscope image for 35 mm H2O2 treated cells, stained with trypan blue dye indicating a cell wall (CW) and cell shrinkage (CS). G–J, Changes in the cellular morphology of H2O2-treated cells analyzed by DAPI nuclear staining, chlorophyll fluorescence and bright-field images. Cells were observed under fluorescence (Left Panels G and I) and bright-field (Right panels H and J) microscopy. Upper (G and H) and lower (I and J) panels correspond to 0 and 24 h of H2O2 treatment, respectively. Apoptotic nuclei are stained in blue dye and, having undergone shrinkage, lack red fluorescence.

Article Snippet: A. thaliana Cell Cultures and PCD Induction A. thaliana MM2d cell suspension cultures (Bayer CropScience) were grown in 1 × Murashige and Skoog (MS) medium (Duchefa Biochemie) supplemented with 30 g/L sucrose (Sigma-Aldrich), 0.5 mg/L NAA (Sigma-Aldrich), 0.05 mg/L kinetin (Sigma-Aldrich), 200 mg/L cefotaxime (Duchefa Biochemie) and 200 mg/L penicillin (Duchefa Biochemie) at 100 rpm and 25 °C.

Techniques: Exclusion Assay, Concentration Assay, Protein Concentration, Bradford Assay, Microscopy, Staining, Fluorescence

BiFC assays in A. thaliana protoplasts. A. thaliana protoplasts were transfected with pSPYCE/pSPYNE vectors, as described in Sheen (34), to corroborate the in vivo interaction of Cc and its potential targets in BiFC. Images were captured 24 h following transient transfection and after 6 h of treatment with 35 mm H2O2. Reconstruction of eYFP leads to the obtainment of green fluorescence signal emission, indicative of interaction between Cc and its partners. Protoplasts transfected with chromatin-remodeling complex element SWI3B, a protein unable to interact with Cc, were used as a negative control. The nucleus was stained in blue using DAPI dye. Scale bar is 10 μm.

Journal: Molecular & Cellular Proteomics : MCP

Article Title: New Arabidopsis thaliana Cytochrome c Partners: A Look Into the Elusive Role of Cytochrome c in Programmed Cell Death in Plants *

doi: 10.1074/mcp.M113.030692

Figure Lengend Snippet: BiFC assays in A. thaliana protoplasts. A. thaliana protoplasts were transfected with pSPYCE/pSPYNE vectors, as described in Sheen (34), to corroborate the in vivo interaction of Cc and its potential targets in BiFC. Images were captured 24 h following transient transfection and after 6 h of treatment with 35 mm H2O2. Reconstruction of eYFP leads to the obtainment of green fluorescence signal emission, indicative of interaction between Cc and its partners. Protoplasts transfected with chromatin-remodeling complex element SWI3B, a protein unable to interact with Cc, were used as a negative control. The nucleus was stained in blue using DAPI dye. Scale bar is 10 μm.

Article Snippet: A. thaliana Cell Cultures and PCD Induction A. thaliana MM2d cell suspension cultures (Bayer CropScience) were grown in 1 × Murashige and Skoog (MS) medium (Duchefa Biochemie) supplemented with 30 g/L sucrose (Sigma-Aldrich), 0.5 mg/L NAA (Sigma-Aldrich), 0.05 mg/L kinetin (Sigma-Aldrich), 200 mg/L cefotaxime (Duchefa Biochemie) and 200 mg/L penicillin (Duchefa Biochemie) at 100 rpm and 25 °C.

Techniques: Transfection, In Vivo, Fluorescence, Negative Control, Staining